论文部分内容阅读
在胞质型水稻雄性不育系IR66707A和IR69700A经离体培养而获得的136个体细胞克隆中,发现了温敏核不育突变5例。这类突变体在广州地区的自然气候下,早季至晚季前期表现为不育,晚季后期表现为可育。盛夏,在幼穗发育至花粉形成阶段对部分突变材料进行短日照处理,发现对短日照敏感的不育系农垦58S转换为可育,而供试的5例突变及另一对照培矮64S却与未经处理的材料一样仍表现为不育,表明它们的育性与日照长度的变化无关。在同一发育时期进行低温处理的结果显示,低温处理10d及10d以上者可发生育性转换,自交结实率在17.23%-42.19%之间,而未经处理的材料仍然表现不育,表明它们的育性转换与温度有关。以正常品种为父本与突变体杂交,F1全部为可育;F2可育与不育个体的分离比为3:1;以F1为父本与之测交,TF1代中可育与不育个体的分离比为1:1。遗传分析表明,这种温敏核不育突变为一对隐性核基因所控制。获得了由胞质型雄性不育变为胞核型雄性不育的突变体,这在体细胞克隆变异领域中是一种典型的突变。
Among 136 somatic cell clones obtained from the in vitro culture of the cytoplasmic male sterile lines IR66707A and IR69700A, five mutations were detected in the thermo-sensitive genic male sterile lines. Under the natural climate in Guangzhou, these mutants showed sterile from early season to early late season and fertile in the late late season. In midsummer, short-day treatment of some mutants was carried out during the stage of panicle development to pollen formation. It was found that Nongken 58S, a sterile line sensitive to short daylight, was transformed into fertile rice. Five of the five mutants and another Pei’ai 64S Like untreated materials, it still showed infertility, indicating that their fertility was not related to the change of sunshine length. The results of low temperature treatment in the same developmental stage showed that fertility transformation could occur after 10 days and 10 days of low temperature treatment, and the selfing rate was between 17.23% -42.19%, while the untreated materials still showed sterility, indicating that they Fertility conversion is related to temperature. The F1 hybrids were fertile and male sterile. The F1 segregation ratio was 3: 1 between F2 fertile and sterile individuals, F1 was the male parent, and F1 was fertile and sterile Individual separation ratio of 1: 1. Genetic analysis shows that this thermo-sensitive nuclear infertility mutation is controlled by a pair of recessive nuclear genes. A mutant that changed from cytoplasmic male sterility to karyotype male sterility was obtained, which is a typical mutation in the field of somaclonal variation.