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目的 :研究 H2 O2 诱导兔晶体上皮细胞凋亡。方法 :采用终浓度 2 5 0 μmol/ L H2 O2 处理兔晶体上皮细胞 ,分别用 TUNEL法、流式细胞仪和透射电镜检测 H2 O2 处理后的兔晶体上皮细胞。结果 :用 H2 O2 处理后的兔晶体上皮细胞 ,TUNEL 法可见胞核呈棕褐色着染 ,苏木素不能复染 ;流式细胞仪检测到约 9.6× 10 5 细胞出现亚二倍体峰 ,二倍体峰几近消失 ;透射电镜下细胞膜完整 ,但表面微绒毛消失 ,细胞质浓缩 ,胞浆内细胞器结构不清晰 ,并见大量大小不等的空泡 ,核质比例增大 ,胞核呈圆形 ,核膜双层结构不清 ,呈光滑状 ,核内染色质聚集成块 ,部分染色质边集。结论 :终浓度 2 5 0μmol/ L H2 O2 可以诱导兔晶体上皮细胞发生凋亡
AIM: To investigate H2O2-induced apoptosis of rabbit lens epithelial cells. Methods: The rabbit lens epithelial cells were treated with 250 μmol / L H 2 O 2 at final concentration. TUNEL, flow cytometry and transmission electron microscopy were used to examine the expression of H 2 O 2 in rabbit lens epithelial cells. Results: TUNEL staining showed that the nuclei of the rabbit lens epithelial cells were stained with TUNEL and stained with hematoxylin. The results of flow cytometry showed that about 9.6 × 10 5 cells appeared sub-diploid peak, The body peak almost disappeared; cell membrane integrity under transmission electron microscopy, but disappeared on the surface microvilli, cytoplasm concentration, intracytoplasmic organelle structure is not clear, and see a large number of different sizes of vacuoles, nuclear ratio increased, the nucleus was round The unclear structure of the nuclear membrane double layer, was smooth, nuclear chromatin gathered into blocks, part of the chromatin edge set. Conclusion: The apoptosis of rabbit lens epithelial cells can be induced by the final concentration of 250 μmol / L H 2 O 2