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Objective To characterize the binding effects of 17D3 anti-pZP monoclonal antibody(mAb) on porcine and human ZP, ovary and other important tissuesMethods The 17D3 anti-pZP mAb was produced by immunizing mouse with porcinezona pellucida and hybridoma and monoclonal antibody preparation. An ABCimmunohistochemistry was used to evaluate reaction of mAb 17D3pZP to porcine andhuman ZP antigens as well as important human tissue antigens.Results mAb 17D3pZP specifically bound to porcine and human ZP antigen, but notto other cells of ovaries and other important human tissue antigens.Conclusion 17D3 anti-pZP mAb can recognize porcine and human ZP antigen withoutcross-reaction with other human tissue antigens including ovary, so it may furtherhelp us to abstract and purify corresponding target antigen and settle basis forproducing human ZP contraceptive vaccine.
Objective To characterize the binding effects of 17D3 anti-pZP monoclonal antibody (mAb) on porcine and human ZP, ovary and other important tissues Methods The 17D3 anti-pZP mAb was produced by immunizing a mouse with porcine zona pellucida and hybridoma and monoclonal antibody preparation. An ABCimmunohistochemistry was used to evaluate the reaction of mAb 17D3pZP to porcine and human ZP antigens as well as important human tissue antigens. Results mAb 17D3pZP bound to porcine and human ZP antigen, but notto other cells of ovaries and other important human tissue antigens. Conlusion 17D3 anti- pZP mAb can recognize porcine and human ZP antigen without cross-reaction with other human tissue antigens including ovary, so it may may furtherhelp us to abstract and purify corresponding target antigen and settle basis forproducing human ZP contraceptive vaccine.