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目的探讨回回甘松饮(HGY)含药血清对高糖诱导的大鼠肾小球系膜细胞(MCs)TGF-β1/Smads信号通路的影响。方法体外培养大鼠MCs,分为正常对照组、高糖组、格列喹酮(阳性)组、HGY高、低剂量组。除正常对照组给外,其余各组分别给予30 mmol/L葡萄糖+10%正常大鼠血清、格列喹酮(10 mg/kg)组大鼠血清、HGY(5、10 g/kg)组大鼠血清干预。细胞培养结束后,采用实时荧光定量PCR方法检测TGF-β1、Smad3、Smad7的基因表达水平。结果高糖(30 mmol/L)使MCs中TGF-β1、Smad3基因表达量升高(P<0.01或P<0.05),使Smad7基因表达量下降(P<0.01);HGY含药血清可下调高糖诱导的MCs中TGF-β1、Smad3的基因表达量(P<0.01),上调Smad7基因表达量下降(P<0.01),且与格列喹酮含药血清的调控结果接近,无显著差异。结论 HGY含药血清可调控MCs在高糖环境下的TGF-β1、Smad3、Smad7的基因表达水平,这可能是其延缓糖尿病肾病进程的机制之一。
Objective To investigate the effects of HGY-containing serum on TGF-β1 / Smads signaling pathway induced by high glucose in rat mesangial cells (MCs). Methods MCs were cultured in vitro and divided into normal control group, high glucose group, gliquidone (positive) group and HGY high and low dose groups. Except for the normal control group, the other groups were given 30 mmol / L glucose + 10% normal rat serum, Gliquidone (10 mg / kg) serum, HGY (5,10 g / kg) Rat serum intervention. After the cell culture was completed, the gene expression levels of TGF-β1, Smad3 and Smad7 were detected by real-time fluorescence quantitative PCR. Results High glucose (30 mmol / L) increased the expression of TGF-β1 and Smad3 in MCs (P <0.01 or P <0.05), and decreased the expression of Smad7 in MCs (P <0.01) The gene expression of TGF-β1 and Smad3 in MCs induced by high glucose (P <0.01) and the up-regulation of Smad7 gene expression decreased (P <0.01), and the results were similar to those of gliquidone-containing serum, showing no significant difference . Conclusions HGY-containing serum can regulate the gene expression of TGF-β1, Smad3 and Smad7 in MCs under high glucose condition, which may be one of the mechanisms that delay the process of diabetic nephropathy.