The structural damage alarming method based on wavelet packet energy spectrum (WPES) for long-span cable-stayed bridges is presented through combination of ambient vibration test and wavelet packet analysis. The environmental variability in the measured WPES and damage alarming indices ERVD of the Runyang Cable-stayed Bridge are discussed in detail using the wavelet packet analysis of the measured acceleration responses of the bridge under daily environmental conditions. The analysis results reveal that the actual environmental conditions including traffic loadings, environmental temperature and typhoon loadings have remarkable correlations with the measured WPES. The changes of environmental temperature have a long-term trend influence on the WPES, while the influences of traffic and typhoon loadings on the measured WPES of the bridge present instantaneous changes because of the nonstationary properties of the loadings. The analysis results of the measured responses further reveal that the damage alarming indices ERVD can sensitively reflect the influences of environmental temperature and typhoon loadings on the dynamic properties of Runyang Cable-stayed Bridge. Therefore, the proposed structural damage alarming indices ERVD under ambient vibrations are suitable for real-time damage alarming for long-span cable-stayed bridges. The structural damage alarming method based on wavelet packet energy spectrum (WPES) for long-span cable-stayed bridges is presented through combination of ambient vibration test and wavelet packet analysis. The environmental variability in the measured WPES and damage alarming indices ERVD of the Runyang Cable-stayed Bridge are discussed in detail using the wavelet packet analysis of the measured acceleration responses of the bridge under daily environmental conditions. The analysis results reveal that the actual environmental conditions include traffic loadings, environmental temperature and typhoon loadings have remarkable correlations with the measured The changes of environmental temperature have a long-term trend influence on the WPES, while the influences of traffic and typhoon loadings on the measured WPES of the bridge present instantaneous changes because of the nonstationary properties of the loadings. The analysis results of the measured responses further reveal tha t the damage alarming indices ERVD can sensitively reflect the influences of environmental temperature and typhoon loadings on the dynamic properties of Runyang Cable-stayed Bridge. Thus, the proposed structural damage alarming indices ERVD under ambient vibrations are suitable for real-time damage alarming for long -span cable-stayed bridges.