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目的探讨血小板第4因子(plateletfactor4,PF4)对5·0Gyγ射线全身照射小鼠的骨髓基质细胞(bonemarrowstromalcells,BMSCs)的保护作用,进一步探讨PF4对造血的辐射防护机制。方法30只雄性小鼠随机分为3组:①放射组,②PF4保护组,③对照组。小鼠照射前分别于26和20h腹腔内注射PF4,每次剂量50μg/kg。于照射后3d取骨髓细胞体外培养,分别计数培养后3、7和14d的骨髓基质细胞集落(CFU-F);在培养后10d流式细胞仪检测细胞周期。结果3组中,照射组3d的CFU-F数量与PF4保护组差异无统计学意义,7和14d的CFU-F数量PF4保护组较照射组明显增加。流式细胞仪检测结果表明3组中照射组G0+G1期细胞明显高于其余两组,S,G2+M期细胞明显低于其余两组。结论PF4对照射小鼠的骨髓基质细胞有保护作用,促进造血重建。
Objective To investigate the protective effect of platelet factor 4 (PF4) on bone marrow stromal cells (BMSCs) induced by 5 Gy γ-rays in mice and to explore the radiation protection mechanism of PF4 on hematopoiesis. Methods Thirty male mice were randomly divided into three groups: ① radiation group, ② PF4 protection group, ③ control group. Mice were injected intraperitoneally with PF4 at 26 and 20 h before irradiation, each at a dose of 50 μg / kg. Bone marrow cells were harvested in vitro three days after irradiation, bone marrow stromal cell colonies (CFU-F) at 3, 7 and 14 days after culture were counted, respectively. Cell cycle was detected by flow cytometry 10 days after culture. Results In the three groups, there was no significant difference in the number of CFU-F between the irradiated group and the PF4-protected group at 3d, and the number of CFU-F group at 7 and 14 days was significantly increased compared with the irradiated group. Flow cytometry results showed that the irradiation group G0 + G1 phase cells were significantly higher than the other two groups, S, G2 + M phase cells was significantly lower than the other two groups. Conclusion PF4 has a protective effect on bone marrow stromal cells in irradiated mice and promotes hematopoietic reconstitution.