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目的考察海洋生物活性碱fascaplysin对肝癌细胞Bel-7402代谢物的影响。方法利用超高效液相色谱-四极杆-飞行时间质谱,采用电喷雾电离源分别在正负离子模式下,对通过fascaplysin处理4、12和24 h的Bel-7402的乙腈提取物进行分析,并通过SIMCA-P软件进行主成分分析和正交偏最小二乘法辨别分析。结果12 h后fascaplysin处理组能够与对照组在PCA得分图上完全区分开,24 h后两组处理则显著分开。分析fascaplysin作用Bel-7402细胞后的潜在代谢标志物,发现主要为磷脂酰乙醇胺、磷脂酰胆碱、鞘磷脂、脂肪酰胺、植物鞘氨醇、二氢神经鞘氨醇、二甲基鞘氨醇、N-Palmitoyl Taurine和N-oleoylTaurine。结论鞘磷脂的下调以及磷脂酰胆碱、植物鞘氨醇、二氢神经鞘氨醇、二甲基鞘氨醇的上调表明胞内鞘磷脂途径被激活可能是fascaplysin引起细胞凋亡的一种作用机制。
Objective To investigate the effect of fascaplysin, a marine bioactive alkali, on the metabolites of Bel-7402 hepatoma cells. METHODS: Acetonitrile extracts of Bel-7402 treated with fascaplysin at 4, 12 and 24 h were analyzed by electrospray ionization (ESI-MS / MS) using ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry. Principal component analysis and orthogonal PLS discrimination analysis by SIMCA-P software. Results After 12 h, the fascaplysin treatment group was completely separated from the control group on the PCA score map. After 24 h, the two treatment groups were significantly separated. The potential metabolic markers of fascaplysin-treated Bel-7402 cells were analyzed and found to be predominantly phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, fatty acid amide, phytosphingosine, sphinganine, sphinganine N-Palmitoyl Taurine, and N-oleoylTaurine. Conclusions The down-regulation of sphingomyelin and the up-regulation of phosphatidylcholine, phytosphingosine, sphinganine, and sphingosine suggest that the intracellular sphingomyelin pathway is activated may be an effect of fascaplysin-induced apoptosis mechanism.