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肝脏是血浆蛋白合成的主要场所。肝病可妨碍许多药物的吸收、血浆蛋白结合和代谢。药物结合蛋白的浓度是药物与血浆蛋白结合程度的决定因素之一。本文作者对16名稳定性肝移植病人(血清总胆红素≤2.2mg/dl,SGOT<40IU/L)、12名健康志愿受试者及7名即将考虑肝移植的晚期肝病病人于头天晚上禁食,抽取10ml新鲜肝素化血液;肝移植病人的血样是在移植后21～146天内获取的。分离血浆,用溴甲酚绿染料结合方法和放射免疫扩散法分别测定比较。两种主要结合蛋白——白蛋白和α_1~-酸性糖蛋白(AAG)的浓度。结果:正常人白蛋白浓度(4.5±0.3g/dl)
The liver is the main site of plasma protein synthesis. Liver disease can hinder the absorption of many drugs, plasma protein binding and metabolism. The concentration of drug-binding protein is one of the determinants of the degree of drug-plasma protein binding. In this study, 16 patients with stable liver transplantation (serum total bilirubin ≤2.2 mg / dl and SGOT <40 IU / L), 12 healthy volunteers and 7 patients with advanced liver disease about to undergo liver transplantation Fasting, 10ml of fresh heparinized blood was drawn; blood samples of liver transplant patients were obtained 21 to 146 days after transplantation. Plasma was separated and compared with bromocresol green dye binding method and radioimmunological diffusion method respectively. Concentrations of two major binding proteins, albumin and alpha 1 - acid glycoprotein (AAG). Results: Normal human albumin concentration (4.5 ± 0.3g / dl)