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目的 通过研究保护性热应激模型建立过程中肿瘤坏死因子 (tumornecrosisfactor,TNF)及一氧化氮 (nitricoxide,NO)的动态变化规律及其作用 ,为该模型的建立提供进一步的理论依据。方法 给予大鼠适量热应激处理 ,然后分离血清 ,分别用放射免疫均相竞争法和硝酸还原酶法测定热应激后 0～ 2 4h不同时相TNF、NO的浓度。结果 热应激后 2、4、8、12h ,TNF浓度升高 ,与对照组比较 ,差异有显著性 (P <0 .0 5、P <0 .0 1) ,0h、2 4h与对照组的差异无显著性 (P >0 .0 5 )。热应激后 4h ,血清TNF浓度达到峰值 [(3.35± 0 .2 0 )ng/ml],较 0h和 2h明显升高 ,差异有显著性 (P <0 .0 1) ,8、12、2 4h较 4h明显降低 ,差异有显著性 (P <0 .0 5、P <0 .0 1) ,2 4h恢复到正常水平。热应激后 2、4、8、12、2 4h ,大鼠血清NO浓度较对照组升高 ,差异有显著性 (P <0 .0 5 )。热应激后 8h ,NO浓度达到峰值 [(10 8.2 1± 2 7.89) μmol/L],与 0、2、4h比较 ,差异有显著性 (P <0 .0 1) ,12h开始下降 ,与 8h比 ,差异有显著性 (P <0 .0 5 ) ,2 4h进一步降低 ,但仍然高于对照组。结论 保护性热应激模型建立过程中TNF、NO起了很重要的作用。
Objective To study the dynamic changes of tumor necrosis factor (TNF) and nitric oxide (NO) during the establishment of protective heat stress model and to provide a theoretical basis for the establishment of this model. Methods The rats were given appropriate amount of heat stress treatment, and then the serum was separated. The concentrations of TNF and NO at different time points after 0 ~ 24 hours of heat stress were determined by radioimmunoassay and nitrate reductase respectively. Results The levels of TNF at 2, 4, 8, 12 h after heat stress were significantly higher than those in the control group (P <0.05, P <0.01), at 0 h and 24 h The difference was not significant (P> 0.05). At 4h after heat stress, the serum TNF concentration reached the peak [(3.35 ± 0.2) ng / ml], significantly higher than 0h and 2h, the difference was significant (P <0.01), 8,12, 2 4h than 4h significantly reduced, the difference was significant (P <0. 05, P <0. 01), 2 4 h returned to normal levels. At 2, 4, 8, 12, 24 h after heat stress, the concentration of NO in rat serum was higher than that in control group (P <0.05). The concentration of NO peaked at 8h after heat stress [(10 8.2 1 ± 2 7.89) μmol / L], compared with 0, 2 and 4 h, the difference was significant (P <0.01) 8h, the difference was significant (P <0. 05), 24h further reduced, but still higher than the control group. Conclusions TNF and NO play an important role in the establishment of protective heat stress model.