【摘 要】
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A fungus (WR-C1) decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then u
【机 构】
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Heilongjiang Economic Research Institute of State Farms, Harbin 150030, China“,”College of Resources
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A fungus (WR-C1) decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then using a filter as the secondary screening medium at low temperature. The experiment showed that the weight loss rate of filter paper on the 15th days could reach 30.69%. A morphologic and ITS gene sequence analysis suggested that CF-C1 wasCladosporium. We mainly studied the effects of culture time, inoculation amount, initial pH and different sources of carbon, nitrogen and inorganic salt on the cellulase production of strain WR-C1. Under optimum cultural condition, the highest value of WR-C1 enzyme production and filter paper enzyme were 3.27 U? mL-1 and 0.51 U? mL-1.
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