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以往的研究表明,200kDa抗原是与吡喹酮协同作用的抗体的靶抗原,通过糖基磷酯酰肌醇(GPI)固着于曼氏血吸虫的表膜上。本文通过加与不加吡喹酮及外源磷酯酶C(PIPLC)进行血吸虫体外培养,观察其表面抗原的释放情况,以验证GPI的存在对吡酮疗效的可能作用。取感染7wk雌鼠体内合抱成虫,经~(85)S-蛋氨酸标记后体外培养于10μg/ml吡喹酮及PIPLC中,以不加PIPLC培养的成虫作为对照。定期收集培养液,100000g离心后备用。用慢性感染小鼠血清,识别血吸虫糖蛋白的单克隆抗体mAb
Previous studies have shown that the 200 kDa antigen is the target antigen of the synergistic antibody to praziquantel and is anchored to the superficial membrane of Schistosoma mansoni by glycosylphosphatidylinositol (GPI). In this paper, Schistosoma japonicum was cultured in vitro with or without praziquantel and exogenous phosphatase C (PIPLC) to observe the release of surface antigens to verify the possible effect of GPI on the efficacy of pyrazinone. Infected 7wk female mice were entrapped in adult worms, labeled with ~ (85) S-methionine in 10μg / ml praziquantel and PIPLC in vitro without adult PIPLC cultured as a control. The culture fluid is collected regularly and centrifuged at 100000g for later use. Monoclonal antibody mAbs that recognize Schistosoma glycoproteins with chronic infection of mouse sera