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作者选择21例未治瘤型麻风病人,随机分成两组,第一天分别给予800mg氟嗪酸(OFLO)或800mg甲氟哌酸(PFLO)顿服,从第7天起至第56天,每天给予400mgOFLO或800mgPFLO。疗前治疗第7,14,28及56天时对各个病人采取皮损活检及血清标本。部分活检标本接种于免疫正常的小鼠和裸鼠,以测定组织中抗酸菌(AFB)活力。接种前测定每毫克湿组织内AFB的数量和形态指数(MI),两者相乘得出每毫克湿组织中所含活菌数量。检测血清中PGL-I时取0.5ml血清,冻干后与氯仿和甲醇混合,经硅胶柱层析提取PGL-I后,在硝
The authors selected 21 patients with untreated leprosy and were randomly divided into two groups. On the first day, either OFLO or 800 mg norfloxacin (PFLO) was given daily. From day 7 to day 56, 400 mg OFLO or 800 mg PFLO per day. Pretreatment treatment on the 7th, 14th, 28th and 56th days of each patient to take skin lesions biopsy and serum samples. Some biopsies were inoculated in immunocompetent mice and nude mice to determine the anti-acid bacteria (AFB) viability in the tissues. The number of AFB and morphometric index (MI) per milligram of wet tissue were measured before inoculation and the two were multiplied to give the number of viable cells per milligram of wet tissue. Serum PGL-I 0.5ml serum was taken, lyophilized mixed with chloroform and methanol after extraction by silica gel column PGL-I, the nitrate