论文部分内容阅读
目的:从基因水平探讨白花蛇舌草多糖对体外培养的人肝癌Bel7402细胞凋亡诱导的作用及机制。方法:水提醇沉法提取白花蛇舌草多糖,人肝癌Bel7402细胞常规体外细胞培养,设定空白对照组、5-氟尿嘧啶阳性对照组、白花蛇舌草多糖组。HE染色法光镜下观察细胞形态改变,计算凋亡指数,采用MTT法检测白花蛇舌草多糖对癌细胞的生长抑制作用,RT-PCR法检测原癌基因bc l-xl、抑癌基因p53基因mRNA表达的变化。结果:白花蛇舌草多糖体外抑制人肝癌Bel7402细胞生长、促进细胞凋亡,凋亡指数达16.97(,以MTT显色法检测细胞抑制率达到61.5(,以RT-PCR半定量法检测,上调p53基因mRNA表达,降低bc l-xl基因mRNA表达,以上各指标与空白对照组相比均有显著差异。结论:白花蛇舌草多糖抑制人肝癌Bel7402细胞增长,诱导细胞凋亡,其分子机制可能与激活抑癌基因p53基因、抑制原癌基因bc l-xl基因表达有关。
OBJECTIVE: To investigate the effect of Hedyotis diffusa Willd polysaccharides on apoptosis induction of human hepatoma Bel7402 cells in vitro and its mechanism. Methods: The conventional cell culture of Hedyotis diffusa, human hepatocellular carcinoma Bel7402 cells was extracted by water extraction and alcohol precipitation. The blank control group, 5-fluorouracil positive control group and Hedyotis diffusa polysaccharide group were set up. The morphological changes of the cells were observed by HE staining and the apoptosis index was calculated. MTT assay was used to detect the inhibitory effect of Hedyotic diffusa on the growth of cancer cells. The expressions of the oncogenes bcl-xl, p53 Changes in gene mRNA expression. Results: The Hedyotis diffusa Willd polysaccharides inhibited the growth of human hepatocellular carcinoma Bel7402 cells in vitro and the apoptosis index was 16.97 (MTT assay showed that the cell inhibition rate reached 61.5 (detected by semi-quantitative RT-PCR, up-regulated p53 gene mRNA expression, reduce bc l-xl gene mRNA expression, the above indicators compared with the blank control group were significantly different.Conclusion: Hedyotis diffusa will inhibit the growth of human hepatoma Bel7402 cells, induce apoptosis, the molecular mechanism It may be related to the activation of the p53 tumor suppressor gene and the suppression of the oncogene bc l-xl gene expression.