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目的采用高效液相色谱(HPLC)法测定氨糖美辛肠溶胶囊中盐酸氨基葡萄糖的含量。方法采用phenomsil BDS C18色谱柱(250nm×4.6mm,5μm),以乙腈-0.01mol/L戊烷磺酸钠溶液(10∶90),检测波长:195nm;进样量:20μl。结果盐酸氨基葡萄糖质量浓度的线性范围为0.015~1.500mg/ml(r=0.9996);平均回收率为99.9%,RSD=0.4%(n=9),精密度为0.76%(n=7),结果良好。结论HPLC法可准确检测出氨糖美辛肠溶胶囊中盐酸氨基葡萄糖的含量。
Objective To determine the content of glucosamine hydrochloride in aminoglymein enteric-coated capsules by high performance liquid chromatography (HPLC). Methods A phenomsil BDS C18 column (250nm×4.6mm, 5μm) was used with acetonitrile-0.01mol/L sodium pentanesulfonate solution (10:90). The detection wavelength was 195nm. The injection volume was 20μl. Results The linear range of glucosamine hydrochloride mass concentration was 0.015~1.500mg/ml (r=0.9996); the average recovery was 99.9%, RSD was 0.4% (n=9), and the precision was 0.76% (n=7). The result is good. Conclusion The HPLC method can accurately detect the content of glucosamine hydrochloride in the aminosulfimin enteric-coated capsules.