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目的了解德国小蠊的谷胱甘肽S-转移酶(GSTs)和乙酰胆碱酯酶(AChE)与抗药性的关系,为探讨德国小蠊抗性机制及建立抗性生化检测方法提供理论依据。方法采用药膜法测定德国小蠊对4种常用杀虫剂的抗性水平,采用分光光度法测定酶活性。结果德国小蠊对DDVP、残杀威、溴氰菊酯和高效氯氰菊酯抗性系数分别为1.08、2.35、7.47和10.92。对德国小蠊抗性和敏感品系GSTs、AChE活力比较结果表明,抗性品系GSTs活力相对于敏感品系明显升高,差异有统计学意义(P<0.01),抗性品系AChE活力相对于敏感品系明显下降,差异亦有统计学意义(P<0.01)。结论德国小蠊抗性品系除对DDVP和残杀威有较弱抗性外,对高效氯氰菊酯产生了高度抗性,对溴氰菊酯产生了一定程度的抗性;对GSTs、AChE活力研究结果表明GSTs的活性增高,使杀虫剂代谢加速,导致杀虫剂对德国小蠊的敏感性下降;AChE作为有机磷和氨基甲酸酯类杀虫药物的靶标酶,其活力下降导致德国小蠊敏感性下降。表明杀虫剂的代谢酶和靶标酶活力的改变,与德国小蠊对有机磷、氨基甲酸酯和菊酯类杀虫剂产生的抗性密切相关。
Objective To understand the relationship between GSTs and acetylcholinesterase (AChE) of German cockroach and its resistance, and to provide a theoretical basis for exploring the resistance mechanism of Blattella germanica and establishing a biochemical detection method. Methods The resistance of Blattella germanica to four commonly used insecticides was determined by the membrane method. The enzyme activity was determined by spectrophotometry. Results The resistance coefficients of Blattella germanica to DDVP, propoxur, deltamethrin and alpha-cypermethrin were 1.08, 2.35, 4.47 and 10.92, respectively. The results showed that the activity of GSTs in resistant and susceptible strains of Blattella germanica was significantly higher than that in susceptible strains (P <0.01), and the activity of AChE in resistant strains was significantly higher than that in susceptible strains Significantly decreased, the difference was statistically significant (P <0.01). Conclusion The resistant strains of Blattella germanica had high resistance to beta-cypermethrin and resistance to deltamethrin, except for DDVP and propoxur. Resistance to GSTs and AChE showed that GSTs increased activity, accelerating the metabolism of insecticides, resulting in decreased sensitivity of insecticides to German cockroach; AChE as a target enzyme of organophosphorus and carbamate insecticides, decreased activity leads to the sensitivity of German cockroach decline. The results showed that the changes of the metabolic enzymes and target enzymes of insecticides were closely related to the resistance of Blattella germanica to organophosphate, carbamate and pyrethroid insecticides.