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目的:观察琐琐葡萄多糖(polysaccharide from Vitis viniferal,VTP)对β-淀粉样蛋白25-35(Aβ25-35)诱导PC12细胞β-淀粉样前体蛋白(beta-amyloid precursor protein,APP)基因表达的影响,探讨VTP在阿尔茨海默病(Alzheimer’s disease,AD)发病机制中的作用。方法:体外培养PC12细胞,分为对照组,AD模型组(20μmol·L-1Aβ25-35作用24 h),VTP低、中、高剂量组质量浓度分别为20,40,80 mg·L-1。VTP作用24 h后,检测细胞活性、超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和细胞超微结构,采用实时荧光定量PCR(real-time PCR)检测APP mRNA表达。结果:与正常组比较,模型组细胞活性及SOD活力降低(P<0.01),MDA含量及APP mRNA表达增高(P<0.01),细胞超微结构明显异常;与模型组比较,给药组细胞活性及SOD活力增强(P<0.05),MDA含量及APP mRNA表达下降(P<0.01),减轻神经细胞损伤。结论:VTP对Aβ25-35诱导的氧化损伤有保护作用,通过抗氧化和调节APP mRNA表达,抑制Aβ形成,保护神经细胞,起到防治AD的作用。
AIM: To investigate the effect of polysaccharide from Vitis viniferal (VTP) on the expression of β-amyloid precursor protein (PC-1) in PC12 cells induced by β-amyloid 25-35 (Aβ25-35) To investigate the role of VTP in the pathogenesis of Alzheimer’s disease (AD). Methods: PC12 cells were cultured in vitro and divided into control group and AD model group (20 μmol·L-1Aβ25-35 for 24 h). The concentrations of VTP in low, medium and high dose groups were 20, 40 and 80 mg · L -1 . After 24-hour VTP treatment, cell viability, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and cell ultrastructure were measured. The mRNA expression of APP was detected by real-time PCR. Results: Compared with the normal group, the cell viability and SOD activity in the model group decreased (P <0.01), the content of MDA and the expression of APP mRNA increased (P <0.01), the ultrastructure of the model group was significantly abnormal. Compared with the model group, Activity and SOD activity (P <0.05), MDA content and APP mRNA expression decreased (P <0.01), and alleviated the damage of nerve cells. CONCLUSION: VTP protects against oxidative damage induced by Aβ25-35. It can prevent and cure AD by inhibiting the expression of APP mRNA, inhibiting the formation of Aβ and protecting nerve cells.