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目前,肾穿刺活检组织的固定方法仍多选择4%中性缓冲甲醛,用该方法固定的肾组织行PAS、PASM染色,能很好地显示肾小球、肾小管、肾间质、血管及特殊的蛋白沉积等,但在Masson染色时,经常会出现嗜复红蛋白不着色或染色很弱的现象。为了解决这个问题,本实验室采用4种方法进行比较,发现组织切片通过补充固定后,用胰酶消化,嗜复红蛋白能很好地显示,并定位准确、背景干净、颜色鲜艳,有助
At present, 4% neutral buffered formaldehyde is still the most commonly used method in renal biopsy, and PAS and PASM staining in renal tissue immobilized by this method can well display glomeruli, renal tubules, renal interstitium, blood vessels and Special protein deposition, etc., but in Masson staining, it is often the case that there is no staining or weak staining of the albumin. In order to solve this problem, our laboratory used four methods to compare and found that tissue sections were fixed by trypsin and digested with trypsin. The results showed that PDGF could be well displayed and its location was accurate, the background was clean, the color was bright,