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利用人喉癌手术切除标本建立了裸鼠移植瘤模型,已传至第9代。原代移植成功率为66％,潜伙期30—70天;鼠间传代移植成功率为100％,潜伏期14—19天,生长稳定。经光学显微镜检查,各代移植瘤组织结构与原人喉癌组织基本一致。电镜检查证明,具有人癌喉特征,癌细胞间有大量桥粒,胞浆中可见较多张力原纤维,有的张力原纤维与桥粒相连。细胞表面有较多指状突起,核膜规则,胞浆中线粒体较多。该移植瘤模型的建立,为研究人喉癌的生物学特性、功能及实验治疗提供了有用的工具。
Nude mice transplantation tumor model was established by surgical resection of human laryngeal carcinoma and passed on to the 9th generation. The success rate of primary transplantation was 66%, the latent period of 30-70 days; success rate of 100% transplanted mice, the incubation period of 14-19 days, stable growth. By optical microscopy, each generation of transplanted tumor tissue and primary human laryngeal carcinoma basically the same. Electron microscopy proved that human larynx laryngeal features, a large number of desmosomes between cancer cells, cytoplasm can be seen more tension fibrils, and some tension fibrils and desmosomes connected. More finger-like protrusions on the cell surface, nuclear membrane rules, more mitochondria in the cytoplasm. The establishment of the xenograft model provides a useful tool for studying the biological characteristics, function and experimental treatment of human laryngeal cancer.