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目的 研究乳腺非典型增生及癌变过程中ＰＣＮＡ及癌基因蛋白ｐ５３、ｃ－ｅｒｂＢ－２的变化规律，并探讨在此过程中细胞增殖活性与ｐ５３、ｃ－ｅｒｂＢ－２癌基因蛋白表达的关系。方法 应用免疫组化法分析了８６例良恶性乳腺组织中的ＰＣＮＡ，癌基因蛋白ｐ５３、ｃ－ｅｒｂＢ－２表达及其相互关系。结果ＰＣＮＡ、ｐ５３、ｃ－ｅｒｂＢ－２的表达随非典型增生程度的增加而增多。ｐ５３、ｃ－ｅｒｂＢ－２阳性表达病例ＰＣＮＣＮ－ＬＩ明显高子阴性病例（ｔ＝２．９０４０，Ｐ＜０．０１；ｔ＝３．２３３３，Ｐ＜０．０１）。结论 ＰＣＮＡ、ｐ５３、ｃ－ｅｒｂＢ－２可反映乳腺癌前病变的恶变倾向，并对癌前病变的临床处理和随访有指导作用；细胞增殖活性与ｒ５３、ｃ－ｅｒｂＢ－２基因激活，蛋白产物聚积有密切联系。
Objective To study the changes of PCNA and oncogene proteins p53 and c-erbB-2 in the process of atypical hyperplasia and carcinogenesis of breast, and to explore the relationship between the cell proliferation activity and the expression of p53 and c-erbB-2 oncogene proteins in this process. Methods The expression of PCNA, oncogene proteins p53 and c-erbB-2 in 86 benign and malignant breast tissues were analyzed by immunohistochemistry. Results The expression of PCNA, p53 and c-erbB-2 increased with the increase of atypical hyperplasia. PCNCN-LI was significantly higher in patients with positive p53 and c-erbB-2 expressions (t=2.9040, P<0.01; t=3.2333, P<0.01). Conclusion PCNA, p53 and c-erbB-2 can reflect the malignant tendency of precancerous lesions of breast cancer, and guide the clinical treatment and follow-up of precancerous lesions; cell proliferation activity and r53, c-erbB-2 gene activation, protein production Accumulation is closely linked.