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目的探讨脑卒中后大鼠边缘系统血管内皮生长因子(VEGF)的演变趋势及益肾调气方药对其早期干预作用。方法选择雄性Wistar大鼠,经Open-Field行为学评分后,随机分为正常组、假手术组、多发脑梗(MCI)组、中药组、西药组。正常组常规饲养。假手术组经颈外动脉推注0.3m L生理盐水,并灌胃蒸馏水。其余各组均采用同种系微栓子体外注入法,由颈外动脉推注同等剂量的同种异体血栓栓子;中药组及西药组在模型制备后,分别予益肾调气方药(6.2 g/kg)、尼莫地平(9.45 mg/kg)干预给药4周。模型复制成功后,于第1、2、4周采用免疫组化检测方法动态观察边缘系统VEGF的变化及中药干预效果。结果 VEGF免疫组化染色结果显示,MCI组VEGF阳性细胞数于第1周明显增多且明显高于其他组,之后逐渐下降。中药组第2、4周VEGF阳性细胞数较MCI组及正常组明显增加。结论脑血管病后大鼠存在边缘系统VEGF的改变,益肾调气方药能加速血管的修复和再生。
Objective To investigate the evolution trend of vascular endothelial growth factor (VEGF) in rat limbic system after stroke and its early intervention by Yishen Tiaogan Recipe. Methods Male Wistar rats were selected and divided into normal group, sham operation group, multiple cerebral infarction (MCI) group, TCM group and western medicine group by Open-Field behavioral score. Normal group of conventional breeding. Sham-operated group was injected 0.3 m L normal saline via external carotid artery, and intragastric distilled water was given. The other groups were injected with the same kind of micro-emboli in vitro, the same dose of allogeneic thromboembolism was injected from the external carotid artery. After the model was prepared, g / kg) and nimodipine (9.45 mg / kg) for 4 weeks. After successful replication of the model, immunohistochemistry was used to observe the changes of VEGF in the marginal system and the effect of Chinese medicine intervention on the 1st, 2nd, 4th week. Results VEGF immunohistochemical staining results showed that the number of VEGF-positive cells in MCI group increased significantly at the first week and was significantly higher than the other groups, then gradually decreased. The number of VEGF-positive cells in Chinese medicine group at 2 and 4 weeks was significantly higher than that in MCI group and normal group. Conclusion There is a change of VEGF in the limbic system in rats after cerebrovascular disease. Yishen Tiaogan prescription can accelerate the repair and regeneration of blood vessels.