论文部分内容阅读
目的 对海南省恶性疟原虫分离株的裂殖子表面蛋白质 1(MSP1)和裂殖子表面蛋白质2 (MSP2 )基因进行分型研究。方法 从海南省恶性疟流行区采集的恶性疟患者血样 ,用套式PCR方法分别扩增PfMSP1和PfMSP2基因中具有型特异性的片段 ,进行等位基因分型。结果 (1)MSP1基因分型 :94份恶性疟患者血样中有 82份扩增出MAD2 0型基因片段 (占 87 2 % ) ,2 7份扩增出K1型基因片段 (占 2 8 7% ) ,15份同时扩增出MAD2 0型和K1型基因片段 (占 16 0 % ) ,未扩增出RO33型基因片段。 (2 )MSP2基因分型 :94份血样中有 75份扩增得到 3D7型基因片段 (79 8% ) ,2 8份扩增得到FC2 7型基因片段 (2 9 8% ) ,10份同时扩增得到 3D7型和FC2 7型基因片段 (占 10 6 % )。结论 海南省恶性疟原虫的MSP1等位基因和MSP2等位基因分别以MAD2 0型和 3D7型为优势基因型 ;不同等位基因型的混合感染率很低
Objective To genotype the MSP1 and MSP2 gene of Plasmodium falciparum isolates from Hainan Province. Methods The blood samples of falciparum malaria patients collected from endemic areas of falciparum malaria in Hainan Province were amplified by nested PCR. The genotypes of PfMSP1 and PfMSP2 were amplified by allele-specific PCR. Results (1) MSP1 genotyping: In 82 of 94 blood samples of patients with P. falciparum, MAD20 gene fragments (87.2%) were amplified and 27 of them were K1 (27.8% ), 15 copies of both MAD20 and K1 (16 0%), and no RO33 gene was amplified. (2) MSP2 genotyping: 75 of the 94 blood samples amplified 3D7 gene fragment (79.8%), 28 amplified fragments of FC2 7 gene (298%), and 10 copies simultaneously expanded Increased 3D7 and FC27 gene fragments (10 6%). Conclusion MSP1 alleles and MSP2 alleles of Plasmodium falciparum in Hainan Province are predominant genotypes of MAD20 and 3D7, respectively. The mixed infection rates of different alleles are very low