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研究狼毒大戟中没食子酸乙酯(ethyl gallate,EG)对人乳腺癌MDA-MB-231细胞侵袭能力的影响,并对其作用机制进行探讨。采用细胞与Matrigel黏附实验,Transwell小室检测EG对人乳腺癌MDA-MB-231细胞黏附、侵袭和运动能力的影响。RT-PCR检测EG对MMP-2、MMP-9的m RNA表达的影响;Western blot法检测EG对Akt-NF-κB信号转导通路蛋白表达的变化。结果显示,EG在体外可显著抑制人乳腺癌MDA-MB-231细胞的侵袭、运动以及黏附能力(P<0.05)。EG可抑制MMP-2、MMP-9的m RNA水平,抑制Akt-NF-κB信号转导通路中的Akt磷酸化和NF-κB蛋白表达。因此认为EG在体外具有一定的抑制乳腺癌细胞侵袭迁移的作用,其机制与抑制MMP-2、MMP-9的m RNA水平、抑制Akt磷酸化过程和NF-κB蛋白表达有关。
To investigate the effect of ethyl gallate (EGG) on the invasiveness of human breast cancer cell line MDA-MB-231 and its mechanism of action. Adherent cells and Matrigel were used to evaluate the effect of EG on the adhesion, invasion and motor ability of human breast cancer MDA-MB-231 cells. The effect of EG on the expression of MMP-2 and MMP-9 mRNA was detected by RT-PCR. The protein expression of Akt-NF-κB signal transduction pathway was detected by Western blot. The results showed that EG could significantly inhibit the invasion, exercise and adhesion of human breast cancer MDA-MB-231 cells in vitro (P <0.05). EG inhibited the m RNA level of MMP-2 and MMP-9 and inhibited Akt phosphorylation and NF-κB protein expression in Akt-NF-κB signal transduction pathway. So that EG in vitro has a certain role in inhibiting the invasion and migration of breast cancer cells, and its mechanism is related to the inhibition of MMP-2, MMP-9 m RNA levels, inhibition of Akt phosphorylation and NF-κB protein expression.