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目的:通过在缺氧条件下体外培养成骨细胞,探讨缺氧对成骨细胞增殖与凋亡的影响以及淫羊藿甙的保护机制。方法:采用酶消化法传代培养乳SD大鼠颅盖骨细胞并建立缺氧模型。实验分2组:组1为单纯缺氧条件下进行的细胞培养,不加入任何药物干预;组2为缺氧条件下加入浓度为10ng/ml的淫羊藿甙与细胞共培养,用台盼蓝染色法分别计数培养5d的细胞数;用流式细胞仪检测2组缺氧5d的细胞凋亡率。结果:缺氧组的细胞数明显少于与淫羊藿甙共培养组,缺氧引起的细胞凋亡率较淫羊藿甙共培养组高。结论:缺氧能抑制成骨细胞的增殖,促进成骨细胞调亡而淫羊藿甙对其具有保护作用。
OBJECTIVE: To investigate the effect of hypoxia on the proliferation and apoptosis of osteoblasts and the protective mechanism of icariine by culturing osteoblasts in vitro under hypoxia. Methods: The SD rat calvarial cells were subcultured by enzyme digestion and hypoxia model was established. The experiment was divided into two groups: Group 1 was cultured under hypoxia alone, without any drug intervention; Group 2 was added hypoxia 10ng / ml concentration of icariin with cells co-cultured with Taiwan hope The number of cells cultured for 5 days was counted by blue staining. The apoptosis rate of the two groups were detected by flow cytometry 5 days after hypoxia. Results: The number of cells in hypoxia group was significantly less than that of icariin co-culture group, and the hypoxia-induced apoptosis rate was higher than icariin co-culture group. Conclusion: Hypoxia can inhibit the proliferation of osteoblasts and promote the apoptosis of osteoblasts. Icariin has a protective effect.