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目的:研究minTBP-PRGDN双靶向融合肽与金属钛表面结合的稳定性及其影响因素。方法:采用FITC标记三种多肽(minTBP-PRGDN、minTBP和PRGDN),在不同离子强度、孵育温度及pH值条件下将多肽溶液分别与钛片孵育过夜,利用荧光显微镜比较分析三种多肽在不同条件下与钛片的结合强度。结果:三种多肽与钛表面均存在结合力,其结合力受离子强度、孵育温度和pH的影响。合适的离子强度有助于多肽在钛表面的结合,在一定范围内,多肽的钛结合能力随着温度的递增而存在一个递减的过程,pH=7.0是多肽与钛表面结合的最适宜pH。结论:minTBP-RGD融合肽不影响minTBP与钛表面的结合能力,4℃、pH=7.0是多肽与钛表面结合适宜环境条件。
OBJECTIVE: To study the stability and its influencing factors of binding of minTBP-PRGDN double-targeting fusion peptide to titanium surface. Methods: Peptide solutions were incubated with titanium plates under different ionic strength, incubation temperature and pH for three days respectively using FITC-labeled three peptides (minTBP-PRGDN, minTBP and PRGDN). Fluorescence microscopy was used to analyze the effect of three peptides Under the conditions of the bonding strength with the titanium. Results: The binding of three peptides to the surface of titanium was affected by the ionic strength, incubation temperature and pH. Appropriate ionic strength contributes to the binding of the polypeptide to the titanium surface. To a certain extent, the binding ability of the titanium decreases with increasing temperature. PH = 7.0 is the optimal pH for the binding of the polypeptide to the titanium surface. CONCLUSION: minTBP-RGD fusion peptide does not affect the binding ability of minTBP to titanium surface. At 4 ℃, pH = 7.0 is suitable for the binding of peptides to titanium surface.