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目的为探讨白藜芦醇是否能成为抗心律失常药,研究了其对窦房结起搏细胞的电生理效应。方法应用细胞内微电极方法记录家兔窦房结起搏细胞的动作电位。结果白藜芦醇(30~120μmol.L-1)显著降低窦房结起搏细胞的动作电位幅度、零相最大上升速率(Vmax)、舒张期除极速率和起搏放电频率。而对最大舒张期电位和90%复极化的时间无明显作用。预先应用L型钙通道开放剂Bay-K-8644(0.5μmol.L-1)灌流窦房结10 min可阻断白藜芦醇(60μmol.L-1)对起搏细胞的上述电生理效应。而应用超极化激活电流阻断剂氯化铯(2 mmol.L-1)加钾通道阻断剂四乙铵(20 mmol.L-1)或应用一氧化氮(NO)合酶阻断剂-LNAME(0.5 mmol.L-1)灌流窦房结标本10min对白藜芦醇(60μmol.L-1)的电生理效应没有明显影响。结论白藜芦醇能抑制家兔窦房结起搏细胞的自发活动,此效应可能与其通过非NO依赖性途径抑制钙离子内流有关。
Objective To investigate whether resveratrol can be an anti-arrhythmic drug and study its electrophysiological effects on pacemaker cells in the sinus node. Methods The intracellular microelectrode method was used to record the action potentials of pacemaker cells in the sinus node of rabbits. RESULTS: Resveratrol (30-120 μmol.L-1) significantly reduced the action potential amplitude, Vmax, diastolic depolarization rate and pacing discharge frequency of sinus node pacemaker cells. There was no significant effect on the maximum diastolic phase potential and 90% repolarization time. The electrophysiological effects of resveratrol (60 μmol.L-1) on pacemaker cells were blocked by pre-applying L-type calcium channel opener Bay-K-8644 (0.5 μmol.L-1) for 10 min. . The use of hyperpolarization-activated current blocker guanidinium chloride (2 mmol.L-1) plus potassium channel blocker tetraethylammonium (20 mmol.L-1) or application of nitric oxide (NO) synthase to block The electrophysiological effects of resveratrol (60μmol.L-1) on the sinus node specimens were not significantly affected by LNAME (0.5 mmol.L-1) perfusion for 10 min. Conclusion Resveratrol can inhibit the spontaneous activity of pacemaker cells in the sinus node of rabbits, and this effect may be related to its inhibition of calcium influx by non-NO dependent pathway.