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目的:探讨麝香、冰片对脑缺血-再灌注损伤大鼠急性期及恢复早期的保护作用及机制。方法:将180只大鼠随机分为假手术组,模型组,麝香高、低剂量组(50、25 mg/kg),冰片高、低剂量组(50、25 mg/kg),醒脑静10m L/kg组。各组大鼠灌胃给药4 d,线栓法复制脑缺血-再灌注模型,观察麝香、冰片对脑缺血-再灌注炎性损伤急性期及恢复早期神经功能缺损评分、脑组织匀浆环氧酶(COX-2)和5脂氧酶(5-LOX)活性变化及海马组织Cys LT2蛋白及mRNA表达的影响。结果:麝香、冰片能显著提高脑缺血-再灌注损伤大鼠神经功能缺损评分,改善其脑组织病理形态,降低脑内COX-2和5-LOX的活性,抑制脑海马组织Cys LT2蛋白表达。结论:麝香、冰片能对抗大鼠脑缺血-再灌注急性期炎性损伤,其机制可能与抑制脑内COX-2与5-LOX的活性有关。
Objective: To investigate the protective effect and mechanism of musk and borneol on acute and early recovery of cerebral ischemia-reperfusion injury in rats. Methods: One hundred and eighty rats were randomly divided into sham operation group, model group, high and low musk density group (50, 25 mg / kg), high and low borneol group 10m L / kg group. The rats in each group were intragastrically administrated for 4 days, and the model of cerebral ischemia - reperfusion was established by the method of thread occlusion. Musk and borneol were used to evaluate the acute phase of cerebral ischemia - reperfusion injury and early recovery of neurological deficit. Changes of COX-2 and 5-LOX Activities and Cys LT2 Protein and mRNA Expression in Hippocampus of Rats. Results: Musk and borneol could significantly improve the neurological deficit score of cerebral ischemia-reperfusion injury rats, improve the pathological changes of brain tissue, decrease the activity of COX-2 and 5-LOX, and inhibit the expression of Cys LT2 in hippocampus . CONCLUSION: Musk and borneol can antagonize the inflammatory injury induced by acute cerebral ischemia-reperfusion in rats. The mechanism may be related to the inhibition of the activity of COX-2 and 5-LOX in the brain.