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目的:了解胃窦胃体联合培养和荧光定量PCR（qPCR）法在临床幽门螺杆菌（Hp）诊断和耐药检测中的应用价值。方法:收集湖州市中心医院2018年7月至2019年3月接受胃镜检查的384例患者胃窦胃体黏膜组织，进行Hp分离培养及常用抗菌药物的药敏试验，并提取患者胃窦处样本的DNA，采用qPCR法进行Hp核酸检测及其对左氧氟沙星和克拉霉素耐药性的检测。结果:胃窦胃体联合培养Hp阳性检出率（147例，38.28%），与qPCR对Hp阳性检出率（164例，42.71%）比较差异无统计意义（n P>0.05）。qPCR对左氧氟沙星和克拉霉素耐药菌的检出率（56.10%和57.93%）均高于联合培养（42.18%和36.73%）（n χ2=6.009和13.950，n P均0.05) . The drug resistance rates of Hp to levofloxacin and clarithromycin by qPCR (56.10% and 57.93%) were higher than those by combined culture (42.18% and 36.73%) (n χn 2=6.009 and 13.950, n P both<0.05) . The inconsistency rates of detection of levofloxacin-resistant and sensitive Hp between qPCR and combined culture method were 43.48% and 36.11%, respectively. The inconsistency rates of detection of clarithromycin-resistant and sensitive Hp were 52.63% and 30.43%, respectively.n Conclusions:The combined culture of gastric antrum and gastric body greatly improves the positive detection rate of Hp, and qPCR method for gastric antrum has superiority in the diagnosis of Hp infection. The qPCR method and the isolation culture method have high inconsistency on drugs resistance of Hp to levofloxacin and clarithromycin, and it should still be relied on the isolation culture method to evaluate the drug resistance of Hp.