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本文旨在研究白细胞介素 2 (interleukin 2 ,IL 2 )对离体大鼠胸主动脉环收缩张力的作用及其可能机制。采用累积加药法 ,检测IL 2对去氧肾上腺素 (PE)和KCl预收缩的胸主动脉环收缩张力的影响。结果表明 ,IL 2 (1、10、10 0、10 0 0U/ml)对PE (10 μmol/L)预收缩的内皮完整血管环产生浓度依赖性的舒张作用 ,而对KCl(12 0mmol/L)预收缩的血管无作用。去除内皮后 ,IL 2的舒张作用被取消。用一氧化氮合酶抑制剂L NAME (0 1mmol/L)和鸟苷酸环化酶抑制剂亚甲蓝 (10 μmol/L)预处理 ,均可阻断IL 2的舒张血管作用。用环氧合酶抑制剂吲哚美辛 (Indo,10 μmol/L)预处理可阻断IL 2的血管舒张作用。从上述观察结果推论 ,IL 2通过NO 鸟苷酸环化酶和环氧合酶途径产生内皮依赖的血管舒张作用
The aim of this study was to investigate the effect and possible mechanism of interleukin 2 (IL 2) on contractile tension of thoracic aorta rings in isolated rat. The effects of IL 2 on contractile tension of thoracic aortic rings pre-contracted with phenylephrine and KCl were examined by cumulative dosing method. The results showed that IL 2 (1,10,10 0,10 0 0U / ml) produced a concentration-dependent relaxation in intact endothelial rings of pre-contracted PE (10 μmol / L) Precontracted blood vessels have no effect. After the removal of the endothelium, the diastolic effect of IL2 was abolished. The preconditioning of nitric oxide synthase inhibitor L NAME (0 1 mmol / L) and guanylyl cyclase inhibitor methylene blue (10 μmol / L) blocked the vasodilatation of IL 2. Pretreatment with cyclooxygenase inhibitor Indometacin (10 μmol / L) blocked the vasorelaxation of IL 2. From the above observations, it is deduced that IL 2 exerts an endothelium-dependent vasodilation through the NO guanylate cyclase and cyclooxygenase pathways