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目的:探讨32P-磷酸铬-聚L乳酸(32P-CP-PLLA)粒子植入H22淋巴转移模型的靶向浓聚及对淋巴转移治疗的潜能和间质植入SD大鼠对其免疫功能的影响。方法:KM小鼠模型50只,随机分为2组,瘤体内分别植入1枚32P-CP-PLLA和注入胶体32P-磷酸铬(32P-CP)。给药后不同时间点处死,观察荷瘤鼠体内生物分布及影像学检查,病理学检查。SD大鼠20只,74 MBq/只,γ相机韧致辐射显像,流式细胞仪动态检测血清CD3、CD4、CD8表达水平。结果:体内生物分布示植入32P-CP-PLLA粒子在瘤体内未发生移位。MR扫描示粒子椭圆形低信号区。γ相机韧致辐射显像示植入粒子组放射性分布持续局限浓聚于植入点。SD大鼠肝脏粒子组免疫力3 d开始降低,7 d最低,14 d恢复正常。肌肉粒子组大鼠免疫力14 d略降低,其余时间正常。肝脏32P-CP组免疫力3 d时降低,7 d降到最低,持续30 d恢复正常。结论:32P-CP-PLLA粒子组织靶向定位明显优于32P-CP,对淋巴转移治疗具有一定的潜能。32P-CP-PLLA植入肌肉组织对机体免疫功能无明显影响,植入肝脏免疫功能一过性轻度降低;同等活度的32P-CP肝脏间质注射可引起机体免疫功能明显损伤,持续约4周左右。
OBJECTIVE: To investigate the potential concentration of 32P-CP-PLLA particles implanted into H22 lymph node metastasis model and its potential for lymph node metastasis and the effects of interstitial SD rats on its immune function influences. Methods: Fifty KM mice were randomly divided into two groups. One 32P-CP-PLLA and 32P-CP were injected into the tumor respectively. After administration, sacrificed at different time points to observe the biodistribution, imaging and pathology of the tumor-bearing mice. 20 SD rats, 74 MBq / mouse, γ-ray bremsstrahlung and γ-ray camera. The expression of CD3, CD4 and CD8 in serum were detected by flow cytometry. RESULTS: In vivo biodistribution showed no translocation of 32P-CP-PLLA particles in the tumor. MR scanning shows the oval low signal area of the particle. γ-ray bremsstrahlung shows that the radioactivity distribution in the implanted particle group continues to be localized at the implantation site. The immunity of SD rat liver particle group began to decrease 3 days, the lowest was at 7 days and returned to normal after 14 days. Muscle particle group rats 14 d slightly reduced immunity, the rest of the time normal. The immunity of the liver of 32P-CP group decreased on the 3rd day and dropped to its lowest level on the 7th day, returning to normal after 30 days. CONCLUSION: The targeted localization of 32P-CP-PLLA particles is better than that of 32P-CP, which has potential for lymphatic metastasis. 32P-CP-PLLA implanted muscle tissue on the immune function had no significant effect, implanted liver immune function transiently decreased slightly; the same activity of 32P-CP hepatic interstitial injection can cause significant damage to the body’s immune function, continued for about About 4 weeks.