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目的筛选出适宜吸附纯化番木瓜籽总三萜的树脂类型,初步确定其吸附-解析工艺条件。方法采用S-8、NAK-9、AB-8、HPD700和D-101 5种不同极性大孔树脂对番木瓜籽总三萜进行吸附纯化,通过比较5种树脂对番木瓜籽总三萜的吸附解析性能及产品回收率,筛选出最佳树脂类型及确定吸附纯化工艺。结果试验表明,相较其他4种树脂,弱极性树脂AB-8较适用于番木瓜籽总三萜的吸附纯化,建立了AB-8静态吸附总三萜的动力学方程,确立了AB-8纯化总三萜的工艺条件,对树脂AB-8的等温吸附进行初步探讨。结论在洗脱剂乙醇体积分数为80%、吸附2 h、解析2 h,AB-8大孔树脂对番木瓜籽总三萜纯化效果较佳,产品的解析率达70.89%,回收率达到59.58%。
OBJECTIVE To screen out the appropriate type of resin for purification of total triterpenoids from papaya seeds and determine the adsorption-desorption conditions. Methods The total triterpenoids of papaya seeds were purified by five different polar macroporous resins S-8, NAK-9, AB-8, HPD700 and D-101. Adsorption and analytical performance and product recovery, screening out the best type of resin and determine the adsorption purification process. The results showed that compared with the other four resins, the weak polar resin AB-8 was more suitable for the purification of the total triterpenes of papaya seeds. The kinetic equation of static adsorption total triterpenoids was established and AB- 8 purification of the total triterpenes process conditions, the isothermal adsorption of resin AB-8 preliminary study. Conclusion The purification of total triterpenoids from papaya seeds by AB-8 macroporous resin is better with the volume fraction of eluted ethanol being 80%, adsorption for 2 h, and resolving for 2 h. The analytical rate of the product is 70.89% and the recovery rate is 59.58 %.