论文部分内容阅读
目的 揭示金属蛋白酶类 (MMPs) ,层粘连蛋白受体 (LN R)与人黑色素瘤细胞侵袭转移的关系 ,并探讨MMPs ,LN R用以判断肿瘤细胞侵袭转移的可能性。方法 通过流式细胞术 (FCM )定量研究和蛋白酶活性分析 (zy mography) ,对具有不同潜在转移能力的人黑色素瘤细胞进行研究。 结果 早期WM 3 5不产生MMPs ;WM 13 4 1B仅产生MMP 2不产生MMP 9;进展期WM 983A和远处转移瘤株WM 45 1既产生MMP 2又产生MMP 9。瘤细胞表面67KDLN R的荧光阳性率和全部细胞的平均荧光强度大小顺序为WM 45 1>WM 983A >WM 13 4 1B >WM 3 5。结论 MMPs和LN R与人黑色素瘤细胞系的侵袭转移能力获得之间存在着密切关系 ,并可作为一种较特异的肿瘤侵袭转移标记物被应用于肿瘤研究与治疗中。
Objective To reveal the relationship between the expression of metalloproteinases (MMPs) and laminin receptor (LN R) and the invasion and metastasis of human melanoma cells, and to explore the possibility of using MMPs and LN R to determine the invasion and metastasis of tumor cells. Methods Human melanoma cells with different potential metastatic potential were studied by flow cytometry (FCM) quantitative studies and protease activity assays (zy mography). Results The early WM 3 5 did not produce MMPs; WM 13 4 1B only produced MMP 2 and did not produce MMP 9; and the advanced WM 983A and distant metastasis strain WM 45 1 both produced MMP 2 and MMP 9. The fluorescence positive rate of 67KDLN R and the average fluorescence intensity of all the cells on the surface of tumor cells were WM 45 1> WM 983A> WM 13 4 1B> WM 3 5. Conclusions There is a close relationship between the expression of MMPs and LN R and the invasion and metastasis of human melanoma cell lines. It may be used as a specific tumor invasion and metastasis marker in cancer research and treatment.