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目的探讨染铅大鼠毒性作用及补硒对铅致损伤的保护作用。方法选取Wistar大鼠40只,随机分为对照组、低高剂量染铅组和硒干预低、高剂量染铅组,每组8只;对照组喂饲基础饲料,低高剂量染铅组在基础饲料中分别添加180、270 mg/kg的醋酸铅,硒干预低、高剂量染铅组分别在低高剂量染铅饲料组中添加0.5 mg/kg亚硒酸钠;7周后处死大鼠检测其体重、脏体比值、血液学、酶学变化及血铅含量等指标。结果给药7周后,高剂量染铅组和硒干预高剂量染铅组大鼠体重分别为(160.22±20.29)和(148.50±9.11)g,均低于对照组的(204.60±26.21)g(P<0.05);除硒干预低剂量染铅组外,肾、脾重量及肝、肾、脾与体重比值均受到影响;低、高剂量染铅组和硒干预高剂量染铅组血红蛋白分别为(12.11±2.06)、(10.24±1.61)和(11.97±1.39)g/dL,均低于对照组的(16.61±4.30)g/dL(P<0.05),硒干预低剂量染铅组为(13.86±2.11)g/dL,与对照组比较差异无统计学意义(P>0.05)。结论选择合适剂量的硒可以对铅致损伤产生保护作用。
Objective To explore the toxic effects of lead-exposed rats and the protective effect of selenium on lead-induced injury. Methods Forty Wistar rats were randomly divided into control group, low-dose lead-exposed group and selenium-treated low and high-dose lead-exposed group, with 8 rats in each group. In the control group, basal diet and low- The basal diet was supplemented with 180,270 mg / kg of lead acetate and selenium with low intervention. High-dose lead-exposed rats were dosed with 0.5 mg / kg sodium selenite in the low-dose lead-exposed group respectively. After 7 weeks, the rats were sacrificed Body weight, dirty ratio, hematology, enzyme changes and blood lead levels and other indicators. Results After 7 weeks of administration, the body weights of high-dose lead-exposed and high-selenium-exposed lead-exposed rats were (160.22 ± 20.29) and (148.50 ± 9.11) g respectively, both lower than those of the control group (204.60 ± 26.21) g (P <0.05). In addition to selenium intervention low dose lead group, the kidney, spleen weight and liver, kidney, spleen and body weight ratio were affected; low and high doses of lead and selenium intervention high-dose lead group hemoglobin Were (12.11 ± 2.06), (10.24 ± 1.61) and (11.97 ± 1.39) g / dL, respectively, which were lower than that of the control group (16.61 ± 4.30) g / dL (13.86 ± 2.11) g / dL, there was no significant difference compared with the control group (P> 0.05). Conclusion Choosing the right dose of selenium can protect against lead-induced injury.