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目的:观察疏血通注射液预防大鼠血管吻合口血栓形成的疗效。方法:将120只Wistar大鼠随机分为对照组、疏血通组及肝素钠组,每组40只。对照组、疏血通组及肝素钠组大鼠分别以生理盐水(10 m L·kg-1)、疏血通注射液(2 g·kg-1)及肝素钠注射液(600 U·kg-1)进行腹腔注射。药物注射6 h后将所有大鼠左侧颈总动脉切断,并即刻对其进行端端吻合。分别于造模结束后30 min及1、3、7 d时从各组随机选取10只大鼠,切取包含吻合口远近端各0.5 cm的颈总动脉,在电子显微镜下观察并计算吻合口及缝线表面覆盖的纤维蛋白、血小板及内皮细胞的百分比。结果:1纤维蛋白覆盖率。造模后不同时间,3组大鼠血管吻合口纤维蛋白覆盖率比较,组间差异均有统计学意义(F=133.802,P=0.000;F=18.741,P=0.000;F=212.943,P=0.000;F=341.174,P=0.000)。造模后30 min时对照组纤维蛋白覆盖率低于疏血通组和肝素钠组(P=0.000,P=0.000);造模后1、3、7 d时对照组纤维蛋白覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000);造模后3 d时疏血通组纤维蛋白覆盖率高于肝素钠组(P=0.000);其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。2血小板覆盖率。造模后不同时间,3组大鼠血管吻合口血小板覆盖率比较,组间差异均有统计学意义(F=352.002,P=0.000;F=84.734,P=0.000;F=368.903,P=0.000;F=14.413,P=0.000)。造模后30 min、1 d、3 d、7 d时对照组血小板覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000);造模结束后30 min时疏血通组血小板覆盖率高于肝素钠组(P=0.027);其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。3内皮细胞覆盖率。除造模后30 min时外,其余各时点3组大鼠血管吻合口内皮细胞覆盖率比较,组间差异均有统计学意义(F=0.000,P=1.000;F=835.502,P=0.000;F=363.874,P=0.000;F=780.410,P=0.000)。造模后1、3、7 d时对照组内皮细胞覆盖率均低于疏血通组和肝素钠组(P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000);造模后1、7 d时疏血通组内皮细胞覆盖率均高于肝素钠组(P=0.002,P=0.001);造模后3 d时疏血通组和肝素钠组比较,组间差异无统计学意义。结论:疏血通注射液能促进内皮细胞生长,减少血小板在血管吻合口的堆积,从而避免血栓形成,其疗效与肝素钠相当。
Objective: To observe the effect of Shuxuetong injection on thrombosis of vascular anastomosis in rats. Methods: 120 Wistar rats were randomly divided into control group, Shuxuetong group and Heparin sodium group, 40 rats in each group. The rats in the control group, Shuxuetong group and Heparin sodium group were treated with normal saline (10 m L · kg -1), Shuxuetong injection (2 g · kg -1) and heparin sodium injection (600 U · kg -1) for intraperitoneal injection. After 6 h of injection, all the left common carotid arteries were excised and anastomosed immediately. Ten rats were randomly selected from each group at 30 min and 1, 3 and 7 d after the model was established. The common carotid arteries containing the 0.5 cm proximal and distal anastomoses were excised and observed under an electron microscope. Suture surface covered by fibrin, platelets and endothelial cells percentage. Results: 1 Fibrin coverage. At different time points after modeling, the coverage of fibrin in vascular anastomosis in the three groups were statistically significant (F = 133.802, P = 0.000; F = 18.741, P = 0.000; F = 212.943, P = 0.000; F = 341.174, P = 0.000). The coverage of fibrin in the control group was lower than that of Shuxuetong group and Heparin sodium group (P = 0.000, P = 0.000) at 30 min after modeling. The coverage of fibrin in the control group at 1, In Shuxuetong group and Heparin sodium group (P = 0.000, P = 0.000; P = 0.000, P = 0.000; P = 0.000, P = 0.000) In the heparin group (P = 0.000). The rest of the time Shuxuetong group and heparin sodium group, no significant difference between groups. 2 platelet coverage. At different time points after modeling, the platelet coverage of vascular anastomotic in the three groups were statistically significant (F = 352.002, P = 0.000; F = 84.734, P = 0.000; F = 368.903, P = 0.000 ; F = 14.413, P = 0.000). The platelet coverage in the control group at 30 min, 1 d, 3 d, and 7 d after modeling was significantly higher than that in Shuxuetong group and Heparin sodium group (P = 0.000, P = 0.000; P = 0.000, P = (P = 0.000, P = 0.000; P = 0.000, P = 0.000). The platelet coverage of Shuxuetong group was higher than that of Heparin group (P = 0.027) at 30 min after modeling. Sodium group comparison, no significant difference between groups. 3 endothelial cell coverage. Except for 30 min after modeling, the coverage of vascular anastomotic endothelium in the other three groups was statistically significant (F = 0.000, P = 1.000; F = 835.502, P = 0.000 ; F = 363.874, P = 0.000; F = 780.410, P = 0.000). The coverage of endothelial cells in the control group at 1, 3 and 7 d after modeling was lower than that of Shuxuetong and Heparin sodium groups (P = 0.000, P = 0.000; P = 0.000, P = 0.000, P = 0.000). After 1 and 7 days of modeling, the endothelial cell coverage of Shuxuetong group was higher than that of heparin sodium group (P = 0.002, P = 0.001). Compared with Heparin sodium group There was no significant difference between groups. Conclusion: Shuxuetong injection can promote the growth of endothelial cells and reduce the accumulation of platelets in vascular anastomosis, so as to avoid thrombosis, and its curative effect is similar to heparin sodium.