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顺钱在孵巢肿瘤化疗中疗效肯定,为进一步探讨其作用机制,本实验采用AO10/17细胞为标本,通过光镜、电镜观察顺铂作用后细胞的形态改变,流式细胞仪(FCM)分析其DNA含量及二苯胺法测定其DNA降解。结果发现20umol/L顺铂作用后24~36h,发生细胞
In order to further explore its mechanism of action, AO10 / 17 cells were used as specimens to observe the morphological changes of cells after cisplatin treatment by light and electron microscope. Flow cytometry (FCM) The DNA content was analyzed and the diphenylamine method was used to determine its DNA degradation. The results showed that 20umol / L cisplatin 24 ~ 36h, the occurrence of cells