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建立了浊点萃取-分光光度法测定灿烂绿的方法。以非离子型表面活性剂Triton X-114为萃取剂,富集、分离灿烂绿,采用分光光度法进行检测。研究了缓冲溶液用量、表面活性剂用量、平衡温度和平衡时间对浊点萃取的影响,得到最佳实验条件:5%的Triton X-114溶液2.5mL、pH3.8的盐酸-柠檬酸钠缓冲溶液2.0 mL、平衡温度和时间分别为50℃和15min。在优化的实验条件下,灿烂绿被萃取到Triton X-114相与水相分离。该方法用于彩笔中灿烂绿的测定,结果满意。
A cloud point extraction and spectrophotometric method for determination of brilliant green was established. With non-ionic surfactant Triton X-114 as extractant, enrichment, brilliant green separation, spectrophotometric detection. The effects of buffer solution dosage, surfactant dosage, equilibrium temperature and equilibration time on the cloud point extraction were investigated. The optimum conditions were as follows: 2.5 mL of 5% Triton X-114 solution, pH3.8 hydrochloric acid-sodium citrate buffer Solution 2.0 mL, the equilibrium temperature and time were 50 ℃ and 15min respectively. Under optimized experimental conditions, brilliant green was extracted to separate the Triton X-114 phase from the aqueous phase. The method is used for bright green determination in crayons, the result is satisfactory.