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目的探讨石棉与烟雾溶液联合作用对人胚肺细胞ＤＮＡ的损伤作用。方法采用非程序ＤＮＡ合成试验，对石棉与烟雾溶液单独及联合作用时人胚肺细胞ＤＮＡ修复合成情况进行了观察。结果石棉与烟溶液单独作用时，均能诱导人胚肺细胞的非程序ＤＮＡ合成试验，且均有明显的剂量反应关系；当二者联合作用时，其造成的细胞３Ｈ标记的胸腺嘧啶核苷（［３Ｈ］－ＴｄＲ）参入量的增加量明显高于二者单独作用时引起的［３Ｈ］－ＴｄＲ参入量的增加量之和。此外，ＯＨ的清除剂二甲基亚砜可部分阻止石棉引起的［３Ｈ］－ＴｄＲ参入。结论石棉与烟溶液联合对人胚肺细胞ＤＮＡ的损伤具有协同作用，ＯＨ在石棉引起的细胞ＤＮＡ损伤中起一定作用。
Objective To investigate the effect of asbestos and aerosol on the DNA damage of human embryo lung cells. Methods Non-procedural DNA synthesis assay was used to observe the DNA repair and synthesis of human embryo lung cells when asbestos and aerosol solution alone and in combination. Results Asbestos and smoke solution alone could induce non-procedural DNA synthesis of human embryo lung cells with significant dose-response relationship. When the combination of both was used, 3H-labeled thymidine ([3H] -TdR) was significantly higher than the increase of the [3H] -TdR incorporation caused by both of them. In addition, OH scavenger dimethyl sulfoxide can partially prevent asbestos-induced [3H] -TdR incorporation. Conclusion The combination of asbestos and smoke solution has a synergistic effect on the DNA damage of human embryo lung cells. OH plays a role in DNA damage caused by asbestos.