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白细胞介素-2(IL-2)通过与其受体(IL-2R)的结合和内在化将信号传递至细胞内,促进T、B细胞的增殖。IL-2R可分为高亲和力受体(H-IL-2R)和低亲和力受体(L-IL-2R),IL-2和H-IL-2R的多寡是影响信号传递的关键因素。有关成年人T细胞该方面的特征已经明确,作为补充,本文对脐带血T细胞表面IL-2R的数目及功能进行了研究。按密度梯度离心法获取成年人外周血单核细胞(PBMC)和脐带血单核细胞(CBMC),调整至规定浓度,分别给与植物血凝素(PHA)和OKT3(后者已与Sepharose 4B偶联)进行刺激。然后作如下检测。
Interleukin-2 (IL-2) promotes the proliferation of T and B cells by signaling to and binding to its receptor (IL-2R) through its internalization. IL-2R can be divided into high-affinity receptor (H-IL-2R) and low-affinity receptor (L-IL-2R), IL- 2 and H-IL-2R is a key factor affecting signal transmission. The characteristics of this aspect of adult T cells have been clarified, and as a supplement, the number and function of IL-2R on the surface of cord blood T cells were studied. Peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cells (CBMCs) were obtained by density gradient centrifugation and adjusted to the prescribed concentrations. Phosphatidylinositol (PHA) and OKT3 Coupling) to stimulate. Then make the following test.