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目的 探讨高危HPV感染与抑癌基因表达产物p5 3、p16蛋白在胃癌发生、发展中的作用及与肿瘤预后的关系。方法 应用原位杂交和免疫组化技术 ,分析 96例胃癌和癌前病变及腺癌组织中HPV 16 /18DNA、p5 3和p16蛋白的阳性表达情况。结果 HPV 16亚型和 18亚型的阳性率分别为 34 38%和 8 33% ;p5 3和p16蛋白阳性表达率分别为 46 88%和 43 75 %。HPV 16亚型、p5 3和p16蛋白在癌前病变中的阳性表达率与腺癌组织有显著差异 (P<0 0 5 ) ,HPV 16和p16蛋白、p5 3蛋白之间具有显著相关性 (P <0 0 5 )。结论 HPV 16亚型感染、p5 3及p16蛋白的水平表达与胃癌的呈正相关 ,p16蛋白的低表达率是肿瘤预后差的标志之一
Objective To investigate the relationship between the high-risk HPV infection and the expression of tumor suppressor gene p53 and p16 proteins in carcinogenesis and progression of gastric cancer and its relationship with tumor prognosis. Methods The positive expression of HPV 16/18 DNA, p5 3 and p16 protein in 96 cases of gastric cancer, precancerous lesions and adenocarcinoma was analyzed by in situ hybridization and immunohistochemistry. Results The positive rates of HPV 16 subtypes and 18 subtypes were 34 38% and 8 33% respectively. The positive rates of p5 3 and p16 protein were 46 88% and 43 75%, respectively. The positive rates of HPV16 subtypes, p5 3 and p16 proteins in precancerous lesions were significantly different from that in adenocarcinoma tissues (P <0.05), and there was a significant correlation between HPV 16 and p16 and p5 proteins P <0 0 5). Conclusions The HPV16 subtype infection, the expression of p5 3 and p16 protein are positively correlated with gastric cancer, and the low expression of p16 protein is one of the markers of poor prognosis