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生物信息学分析发现西(甜)瓜果斑病菌(Acidovorax citrulli,Ac)基因组中存在水稻条斑病菌(Xanthomonas oryzae pv.oryzicola,Xoc)avrRxo1的同源基因avrRxo1_(Ac)。为了揭示avrRxo1_(Ac)是否能够激发玉米R基因Rxo1介导的过敏反应(Hypersensitive response,HR),本研究克隆了avrRxo1_(Ac)基因;氨基酸同源性分析结果显示:AvrRxo1_(Ac)与AvrRxo1的同源性为53.16%。将avrRxo1_(Ac)置于Xoc的avrRxo1突变体中,发现其不能恢复该突变体在含有Rxo1玉米上激发HR反应的能力;借助农杆菌瞬时表达体系发现AvrRxo1A,也不能在Rxo1玉米上激发HR反应。这些结果表明avrRxo1_(Ac)-Rxo1互作不表现为基因-对-基因关系,暗示Rxo1抗病基因转入西甜瓜中,将不能起到抗西(甜)瓜细菌性果斑病的作用。
Bioinformatics analysis showed that the homology gene avrRxo1_ (Ac) of Xanthomonas oryzae pv.oryzicola (Xoc) avrRxo1 was found in the genome of Acidovorax citrulli (Ac). In order to reveal whether avrRxo1_ (Ac) can stimulate the Rxo1-mediated hypersensitive response (HR) of maize R gene, avrRxo1_ (Ac) gene was cloned in this study. The results of amino acid homology analysis showed that the interaction between AvrRxo1_ (Ac) and AvrRxo1 The homology was 53.16%. AvrRxo1_ (Ac) was placed in the avrRxo1 mutant of Xoc and was found to not be able to restore its ability to elicit HR responses on Rxo1-containing maize; AvrRxo1A was found by Agrobacterium transient expression system and also failed to elicit HR responses on Rxo1 maize . These results indicated that the avrRxo1_ (Ac) -Rxo1 interaction did not show the gene-to-gene relationship, suggesting that the transformation of the Rxo1 resistance gene into cantaloupe would not play a role in resistance to Western blotting.