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目的：研究载脂蛋白E基因多态性对丹参素调脂作用的影响。方法用逆转录聚合酶链反应法检测不同基因型载脂蛋白E与丹参素共同作用于L－02细胞后，L－02细胞低密度脂蛋白受体和羟甲戊二酰辅酶A还原酶mRNA表达的变化。结果 ApoE 能够明显减弱丹参素对 LDLR mRNA 表达的上调（ P＜0.01），突变型E2（ Arg112 Cys ）减弱强度明显大于野生型E3和突变型E4（ Cys 158 Arg，P＜0.01）；ApoE亦能够增强丹参素对羟甲戊二酰辅酶A还原酶mRNA表达的下调， E4增强作用最大（ P ＜0.01），但 E2、E3作用不明显（ P＞0.05）。结论载脂蛋白E 112和158位均可能是影响丹参素调脂作用的关键位点。“,”Objective To study the action of apolipoprotein E genetic polymorphism on the efficacy of Tanshinol.Methods Through detecting the expression of low density lipoprotein receptor mRNA and 3-hydroxy-3 -methylglutaryl coenzyme A reductase mRNA in L -02 cells by reverse transcription-polymerase chain reaction , to compare the effect of different genetic subtypes of ApoE on Tanshinol.Results Apolipopro-tein E can weaken the induction of low density lipoprotein receptor ex-pression caused by Tanshinol.The weakening extent of apolipoprotein E 2 (Arg112Cys)was stronger than apolipoprotein E3 and apolipoprotein E4 ( Cys 158 Arg) ( P<0.01 ).Apolipoprotein E also can enhance the inhi-bition of 3-hydroxy -3-methylglutaryl coenzyme A reductase expres-sion, especially apolipoprotein E4 (P0.05 ).Conclusion Both 112 and 158 site might be the key site that have effect on the efficacy of Tanshinol.