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目的通过对雄性新西兰兔精液中精子质量及受精能力的检测,观察睾丸大体形态及精子细胞超微结构的改变,探讨局部振动对雄性兔的生殖功能的影响。方法将新西兰兔随机分为A组(接振强度为3.02m/s~2)、B组(接振强度为6.13m/s~2)、C组(接振强度为12.26m/s~2)和1个对照组。于接振后30d测定精浆中精子的密度、活动力、活动率、畸形率,通过去透明带仓鼠卵-精子穿透试验和精子低渗膨胀试验评价精子的受精能力,光镜下观察睾丸组织学变化,电镜下观察睾丸精子细胞超微结构的改变。结果接振试验后与对照组相比较,A、B、C 3个试验组新西兰兔精液精子活动率、活动力明显降低(P<0.01);精子的畸形率明显升高(P<0.05)。精子的受精率及尾部肿胀的精子比率明显降低(P<0.05)。睾丸生精细胞数量减少,精子细胞的部分细胞器破坏、裂解、坏死。结论后肢接振试验可降低精子的活动力、活动率及卵子受精率及低渗肿胀率,增加精子的畸形率,睾丸组织中生精细胞减少,精子细胞的部分细胞器破坏、裂解、坏死,从而抑制精子的成熟,影响生殖功能。
OBJECTIVE: To observe the changes of sperm morphology and sperm ultrastructure in sperm of male New Zealand rabbit semen, and to investigate the effect of local vibration on the reproductive function of male rabbits. Methods New Zealand white rabbits were randomly divided into group A (vibration intensity 3.02m / s ~ 2), group B (vibration intensity 6.13m / s ~ 2), group C (vibration intensity 12.26m / s ~ ) And 1 control group. The sperm density, motility, activity rate and malformation rate in seminal plasma were measured 30 days after resuscitation, the sperm fertilization ability was evaluated by the hamster egg-sperm penetration test and sperm hypotonic expansion test in the zona pellucida, and the testis Histological changes were observed under electron microscope ultrastructural changes of testicular sperm cells. Results Compared with the control group, the motility and sperm motility of New Zealand rabbit semen in A, B and C groups were significantly decreased (P <0.01). The abnormality rate of sperm was significantly increased (P <0.05). The rate of sperm fertilization and swelling of the tail sperm was significantly lower (P <0.05). Testicular spermatogenic cells reduce the number of sperm cells organelles destruction, lysis, necrosis. Conclusion The hind limb vibration test can reduce sperm motility, activity rate, fertilization rate and hypoosmolar swelling rate, increase the rate of sperm deformity, spermatogenic cells in testes, destruction of some organelles, lysis and necrosis of sperm cells Inhibit the maturation of sperm, affecting reproductive function.