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目的检测凋亡抑制因子5(API5)在卵巢癌中的表达,分析其与临床病理特征的关系。方法Western blotting方法检测2例正常卵巢组织及6例不同级别的卵巢癌组织的表达,组织化学方法检测10例正常卵巢、119例卵巢癌(浆液性腺癌61例、黏液性癌5例、内膜样癌10例、透明细胞癌10例及未分化癌33例)组织中API5的表达,分析其表达与临床病理参数及5年生存率之间的相关性,细胞转染及凋亡流式细胞分析检测API5及顺铂对卵巢癌细胞的作用。结果 API5在卵巢癌组织中的表达高于正常卵巢组织,API5的表达与临床病例特征之间密切相关,Kaplan-Meier生存曲线揭示API5表达高的卵巢癌患者较低表达患者生存率低,流式细胞术分析显示,抑制API5的表达能增加顺铂对卵巢癌细胞的敏感性。结论 API5的异常表达与卵巢癌的发生密切相关,提示API5在预后评价中具有潜在的临床应用价值,有可能成为卵巢癌治疗的潜在靶点。
Objective To detect the expression of apoptosis inhibitor 5 (API5) in ovarian cancer and analyze its relationship with clinicopathological features. Methods Western blotting was used to detect the expression of 2 normal ovarian tissues and 6 different grades of ovarian cancer tissues. Ten normal ovarian tissues and 119 ovarian carcinomas were detected by histochemical staining (61 cases of serous adenocarcinoma, 5 cases of mucinous carcinoma, 10 cases of carcinoma, 10 cases of clear cell carcinoma and 33 cases of undifferentiated carcinoma). The correlation between the expression of the protein and clinicopathological parameters and 5-year survival rate was analyzed. The results of cell transfection and flow cytometry Analysis of the detection of API5 and cisplatin on ovarian cancer cells. Results The expression of API5 in ovarian cancer tissues was higher than that in normal ovarian tissues. The expression of API5 was closely related to the clinical features. The Kaplan-Meier survival curve revealed that patients with low expression of API5 had lower survival rates, Cytometric analysis showed that inhibition of API5 expression increased the sensitivity of cisplatin to ovarian cancer cells. Conclusion The abnormal expression of API5 is closely related to the occurrence of ovarian cancer, suggesting that API5 has potential clinical value in the prognosis evaluation and may become a potential target for the treatment of ovarian cancer.