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目的 探讨前列腺素E1对链尿佐菌素诱导的 1型糖尿病大鼠肾组织肝细胞生长因子 (HGF)的调节。方法 将大鼠随机分为正常对照组、糖尿病模型组和前列腺素E1(PGE1)治疗组。于模型成功后第 7、14、2 8天分别取各组 5只大鼠 ,检测肾功能和组织病理改变 ,用免疫组化法检测肾小球和肾小管 -间质中HGF的表达 ,并用ELISA法比较各组肾组织分泌的HGF。结果 PGE1治疗 7、14、2 8天时 ,治疗组HGF的表达及分泌均较糖尿病模型组增高 ,并于第 14天达高峰。结论 PGE1可以上调 1型糖尿病大鼠肾组织HGF的分泌 ,加强HGF的肾保护作用 ,延缓糖尿病肾病的进展
Objective To investigate the regulation of prostaglandin E1 on hepatocyte growth factor (HGF) in streptozotocin-induced type 1 diabetic rats. Methods The rats were randomly divided into normal control group, diabetic model group and prostaglandin E1 (PGE1) treatment group. On the 7th, 14th and 28th day after the model was successfully established, 5 rats in each group were taken to detect the renal function and histopathological changes. The expression of HGF in glomeruli and tubulointerstitium was detected by immunohistochemistry The HGF secreted by renal tissues in each group was compared by ELISA. Results When PGE1 was administered for 7, 14 and 28 days, the expression and secretion of HGF in the treatment group were higher than those in the diabetic model group, reaching the peak on the 14th day. Conclusion PGE1 can up-regulate the secretion of HGF in renal tissues of type 1 diabetic rats, enhance the renal protective effect of HGF and delay the progression of diabetic nephropathy