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目的:建立一种测定板蓝根颗粒中表告依春含量的方法,并比较不同厂家板蓝根颗粒中表告依春的含量差异。方法:应用高效液相色谱法测定表告依春的含量,色谱柱为ZORBAXSB-C18(4.6mm×150mm,5μm);流动相为乙腈-水-磷酸-三乙胺(8.5:90.72:0.73:0.05);流速为0.7ml/min;检测波长为245nm;进样量为20μl;柱温为30℃。结果:表告依春含量测定的线性范围为0.0204-0.3060μg(r=0.9998),平均加样回收率为98.08%,RSD为1.25%。结论:本研究所建立的色谱方法可以简便、准确测定板蓝根颗粒中表告依春的含量,重现性好;各厂家生产的板蓝根颗粒中表告依春的含量差别较大,有必要建立表告依春的定量控制方法。
OBJECTIVE: To establish a method for determining the content of epirubicin in Radix isatidis and to compare the contents of epirubicin in Radix isatidis from different manufacturers. METHODS: The content of Erianthoxylisochrysalis was determined by HPLC. The chromatographic column was ZORBAXSB-C18 (4.6mm × 150mm, 5μm). The mobile phase consisted of acetonitrile-water-phosphoric acid- triethylamine (8.5: 90.72: 0.73: 0.05). The flow rate was 0.7ml / min. The detection wavelength was 245nm. The injection volume was 20μl. The column temperature was 30 ℃. Results: The linear range was 0.0204-0.3060μg (r = 0.9998). The average recoveries were 98.08% and the RSD was 1.25%. Conclusion: The chromatographic method established in this study can be easily and accurately determine the content of Erianthocyanidins in Banlangen granules with good reproducibility. The contents of Erianthogenol in Banlangen granules produced by different manufacturers are quite different, so it is necessary to establish a table According to the quantitative control method of spring.