作者以人宫颈癌DNA经二轮转染NIH/3T3细胞,得到明显转化灶,将转化细胞注入裸鼠,形成纤维肉瘤。经分子杂交证明,二轮转化细胞均可检测到人顺序,说明人顺序巳转移至小鼠细胞内,并呈恶性转化。将转化细胞及裸鼠肿瘤DNA经Bam HI酶切后,与~(32)P标记的Ha-ras探针杂交呈6.6kb的专一区带,说明人宫颈癌癌基因与Ha-ras具有同源顺序。 The authors transfected NIH/3T3 cells with human cervical cancer DNA in two rounds to obtain a distinct focus of transformation. The transformed cells were injected into nude mice to form fibrosarcoma. Through molecular hybridization, it was demonstrated that the human transformation sequence was detected in the second round of transformed cells, indicating that the human sequence was transferred to mouse cells and that it was malignantly transformed. The transformed cells and the tumor DNA of nude mice were digested with Bam HI and hybridized with a (32)P-labeled Ha-ras probe and showed a specific band of 6.6 kb, indicating that the oncogene of human cervical cancer has the same characteristics as Ha-ras. Source order.