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目的 :观察大鼠脑缺血 /再灌注后脑细胞单、双链DNA损伤情况 ,以揭示该病的损伤机制。方法 :分别用Klenow及TdT(TUNEL)标记染色法检测大脑中动脉阻塞 (MCAO)模型组、假手术组及正常大鼠脑细胞的单、双链DNA断裂 ,观察缺血侧、对照侧皮质及海马阳性细胞数的动态变化。结果 :MCAO模型组缺血侧皮质及海马Klenow及TUNEL阳性细胞数均逐渐增多 ,Klenow阳性细胞的出现早于TUNEL阳性细胞 ,皮质的出现早于海马。结论 :大鼠MCAO模型中DNA单、双链断裂均参与了脑损伤机制
Objective: To observe the single and double-stranded DNA damage of brain cells after cerebral ischemia / reperfusion in rats to reveal the mechanism of the injury. Methods: The single and double stranded DNA of MCAO model group, sham operation group and normal rat brain were detected by Klenow and TdT (TUNEL) staining respectively. The ischemia and control cortex Dynamic changes of positive hippocampal cells. Results: The number of Klenow and TUNEL-positive cells in ischemic cortex and hippocampus increased gradually in MCAO model group. The appearance of Klenow positive cells was earlier than TUNEL positive cells, and the appearance of cortex appeared earlier than hippocampus. Conclusion: Single and double strand breaks in rat MCAO model are involved in the mechanism of brain injury