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[目的]探讨体内外氩氦刀靶向治疗对肝癌细胞灭活作用的影响因素及联合高渗盐水提高冷冻疗效的可行性。[方法]通过氩氦刀对体外培养的人肝癌SMMC7721细胞予不同速度(快冻或慢冻)、低温(-40℃以下或以上)和冻融周期(1个或2个周期)冷冻处理。H22肝癌荷瘤小鼠随机分为5组:Ⅰ组为根治冷冻组,Ⅱ组为姑息冷冻组,Ⅲ组为高渗盐水注射组,Ⅳ组为姑息冷冻联合高渗盐水治疗组,Ⅴ组为无治疗组。[结果]冷冻温度低于-40℃时,细胞死亡率可达100%。冷冻温度为-40℃~-20℃时,细胞死亡不完全。Ⅰ、Ⅱ、Ⅲ、Ⅳ组均能显著抑制肿瘤生长,抑瘤率分别为99.7%、83.6%、78.9%、99.3%;以上各组与Ⅴ组比较,差异均有显著性。Ⅰ组与Ⅳ组比较差异无显著性。[结论]快速冷冻、重复冻融、-40℃以下低温可完全杀灭人肝癌细胞。冷冻与高渗盐水联合治疗是一种有效的肝癌综合治疗模式。
[Objective] To explore the factors that affect the inactivation of hepatocarcinoma cells in vitro and in vivo and the feasibility of enhancing the curative effect by combining with hypertonic saline. [Method] The human hepatocellular carcinoma SMMC7721 cells cultured in vitro were cryopreserved by cryogenic temperature (-40 ℃ or below) and freeze-thaw cycles (1 or 2 cycles) by using argon-helium knife. H22 hepatocarcinoma tumor-bearing mice were randomly divided into five groups: group I was a radical freezing group, group II was palliative freezing group, group III was hypertonic saline injection group, group IV was palliative freezing combined with hypertonic saline group, group V was No treatment group. [Results] When the freezing temperature was lower than -40 ℃, the cell death rate could reach 100%. When the freezing temperature is -40 ℃ ~ -20 ℃, cell death is incomplete. Group Ⅰ, Ⅱ, Ⅲ and Ⅳ all could significantly inhibit the growth of tumor. The tumor inhibition rates were 99.7%, 83.6%, 78.9% and 99.3% respectively. The difference between the above groups and group V was significant. There was no significant difference between group Ⅰ and group Ⅳ. [Conclusion] Rapid freezing, repeated freezing and thawing, low temperature below -40 ℃ can completely kill human hepatoma cells. Frozen and hypertonic saline combination therapy is an effective treatment of liver cancer model.