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目的采用HPLC法测定清热止咳颗粒中贝母素甲和贝母素乙的含量。方法色谱柱为Agilent Eclipse XDB C18(250mm×4.6 mm,5μm),流动相为乙腈-水-二乙胺(70∶30∶0.03),流速1.0 m L·min-1,柱温30℃,ELSD漂移管温度为70℃,增益为4。结果贝母素甲的线性范围为0.906~4.53μg(r=0.9994),加样回收率为98.93%,RSD=0.59%(n=6);贝母素乙的线性范围为0.817~4.085μg(r=0.9993),加样回收率为98.28%,RSD=0.97%(n=6)。结论所用方法简便、准确、快速,重复性好,适用于清热止咳颗粒的质量控制。
Objective To determine the content of peimine and peiminine B in Qingre Zhike Granules by HPLC. Methods The column was Agilent Eclipse XDB C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-water-diethylamine (70:30:0.03) and the flow rate was 1.0 m L · min-1. Drift tube temperature is 70 ° C and gain is 4. Results The linear range of peiminine A was 0.906 ~ 4.53μg (r = 0.9994), the recovery rate was 98.93% and the RSD was 0.59% (n = 6). The linear range of peiminine B was 0.817 ~ 4.085μg r = 0.9993). The recovery rate was 98.28% with RSD = 0.97% (n = 6). Conclusion The method is simple, accurate, rapid and reproducible. It is suitable for the quality control of Qingre Zhike Granule.