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选用两个具有抗性差异的玉米自交系B73与Mo17,分别对其根部进行干旱、脱落酸(ABA)、茉莉酸甲酯(MeJA)以及茉莉酸甲酯与乙烯利联合(MeJA/EP)处理,定量检测4个与防御相关的萜类合酶基因An2、ZmTPS6、ZmTPS8以及ZmTPS26的表达情况。结果表明,4个基因在B73与Mo17应对干旱和ABA处理时基因表达差异明显。在Mo17中,干旱胁迫以及ABA处理显著诱导4个基因的表达;在B73中,干旱处理导致An2、ZmTPS6和ZmTPS8基因表达下调,在ABA处理下时,只有ZmTPS6被显著上调;MeJA与MeJA/EP处理在B73与Mo17中都能上调4个基因的表达,但在Mo17中的诱导程度更剧烈。B73与Mo17的根部在响应胁迫时存在显著的萜类代谢差异,可能为其抗性差异原因之一。
Two maize inbred lines B73 and Mo17 with different resistance were selected to conduct drought, abscisic acid (ABA), methyl jasmonate (MeJA) and methyl jasmonate (MeJA / EP) The expression of four defense-related terpenoid synthase genes, An2, ZmTPS6, ZmTPS8 and ZmTPS26, were detected and quantified. The results showed that there were significant differences in gene expression among the four genes in response to drought and ABA treatment. In Mo17, the expression of four genes was significantly induced by drought stress and ABA treatment. In B73, the expression of An2, ZmTPS6 and ZmTPS8 genes was down-regulated under drought stress. Only ZmTPS6 was up-regulated under ABA treatment. MeJA and MeJA / EP Treatment both up-regulated the expression of four genes in B73 and Mo17, but induced more strongly in Mo17. There were significant differences in terpenoid metabolism between the roots of B73 and Mo17 in response to stress, which may be one of the reasons for their differences in resistance.