论文部分内容阅读
本文证明无菌培养基内生长的痢疾阿米巴在体外释放排泄一分泌抗原(ESA)。用免疫血清和痢疾阿米巴病人血清来阐明ESA的免疫化学反应性。培养的痢疾阿米巴(NIH:200)在改良的Diam-onds TPS—1培养基内无菌地生长。粗制可溶性抗原(CSA),由培养基内收获的阿米巴按Das等的方法制备。即将滋养体超声处理后,匀浆以6000×g转速离心30分钟,冰冻干燥上清液,在4℃储存备用。使用时用适量生理盐水恢复冻干抗原的水分,用Lowry等的方法测定抗原的蛋白质含量。痢疾阿米巴(NIH:200))滋养体在培养30,48和72小时后收获,以研究在不同生长期内在上清液里释放的
This paper demonstrates that excretion of a secreted antigen (ESA) in vitro by amoebae dysentery grown in sterile medium. Immuno-chemical reactivity of ESA was elucidated with serum from immunized sera and amoebic dysentery patients. The cultured dysentery amoebae (NIH: 200) grew aseptically in modified Diam-onds TPS-1 medium. Crude soluble antigen (CSA), prepared from the amoeba harvested in the medium, was prepared by the method of Das et al. After the trophozoites were sonicated, the homogenate was centrifuged at 6000 × g for 30 minutes and the supernatant was lyophilized and stored at 4 ° C until use. When using the amount of normal saline with water to restore the lyophilized antigen, using Lowry and other methods to determine the antigen protein content. Diarrhea trophozoites were harvested at 30, 48, and 72 hours after culture in order to study the release of trophozoites in the supernatant at different growth stages